Chemical evidence for associated TRF with subcellular fractions after incubation of intact rat prolactin cells (GH3) with 3 H-labelled TRF

On a roll for new TRF targets.

Molecular Recognition in Complexes of TRF Proteins with Telomeric DNA

Telomeres are specialized nucleoprotein assemblies that protect the ends of linear chromosomes. In humans and many other species, telomeres consist of tandem TTAGGG repeats bound by a protein complex known as shelterin that remodels telomeric DNA into a protective loop structure and regulates telomere homeostasis. Shelterin recognizes telomeric repeats through its two major components known as ...

Pre-incubation of subcellular fractions from rat cerebral cortex inactivates protein phosphorylation.

Pre-incubation of various subcellular fractions from rat brain caused a significant decrease in the phosphorylation of individual polypeptides. The rate and extent of this loss of labelling was not uniform and polypeptides whose phosphorylation was independent of cyclic AMP were primarily affected, whereas substrate availability remained unaltered. It is recommended that pre-incubation effects ...

Effect of antimicrotubule agents on terminal glycosyltransferases and other enzymes associated with rat liver subcellular fractions.

Previous studies have shown that anti microtubule agents disrupt Golgi complexes in hepatocytes and other cells, causing breakdown or vesiculation of Golgi cisternal membranes. Whether this change in the structure of the Golgi membranes is associated with changes in Golgi membrane function is not known. The present study was initiated to investigate this issue; i.e., to determine whether anti-m...

TRF concentrations in various tissues following anterior hypothalamic lesions.

TRF(Thyrotrophin-Releasing-Factor) is produced in the anterior hypothalamus, deposited in the neurohypophysis, and then passed into the cerebrospinal fluid and the blood stream to be excreted into urine(Shibusawa et al., 1959c). It has been demonstrated neither in the median and posterior hypothalamus, nor in other tissues, and body fluids. It is an hitherto unknown hormone which controls the t...